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In contrast, heavy-ion beam irradiation has emerged as a potent physical mutagen, with distinct advantages in plant breeding and microbial engineering ( 12 C 6+ ) exhibit high linear energy transfer (LET) and a defined penetration depth, generating dense ionization tracks that induce complex, clustered DNA damage—including double-strand breaks (DSBs) ( Although 12 C 6+ heavy-ion irradiation is well established in cancer therapy and materials science, its systematic application in animal cell engineering, particularly for targeted modification of host cells to alter viral replication efficiency, remains underexplored
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[Google Scholar] 41.Huang CH, Sung HC, Hsiao CY, et al